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Antibody(plasma membrane)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(plasma membrane)

【A】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
mouse Acinar-12011-08-10 WBIF IHCIP
DSHB
3.7A12
rat

試してない?
Not used
ASAHー22011-08-10 WBIF IHCIP
Bio visionx100
3828-100
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
mouse pan-AMPAR2011-08-10 WBIF IHCIP
Frontier sci.x100
panAMPAR-GPAf5801
3%PFA (in PBS)パパインの前処理必要
Pretreatment by papain is required
guinea pig
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
human AQP12011-08-10 B-11WBIF IHCIP
Santa Cruzx100
sc25287
3%PFA (in PBS), methanol
mouse
m

マウス腎臓
rat AQP12011-08-10 1WBIF IHCIP
Santa Cruzx100
sc32738
3%PFA (in PBS), methanol
mouse
m

マウス腎臓
human AQP12011-08-10 C-20WBIF IHCIP
Santa Cruzx100
sc9879
3%PFA (in PBS)
goat
p

マウス腎臓
human AQP22011-08-10 C-17WBIF IHCIP
Santa Cruzx100
sc9882
3%PFA (in PBS)
goat
p

マウス腎臓
mouse CD132011-08-10 aminopeptidaseWBIF IHCIP
BMAx100
T2015
3%PFA (in PBS)
rat
p

マウス小腸

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
CD42011-08-29 YNB46.1.8WBIF IHCIP
serotecx100
rat
Mono, IgG1

Hela細胞
CD42011-08-29 AF-379-NAWBIF IHCIP
R&D SYSTEMSx10
goat
poly
取り込み実験には厳しい
Not suitable for uptake study.
Hela細胞
CD4-Alexa4882011-08-29 RPA-T4WBIF IHCIP
BioLegendx50
mouse
mono, IgG1

Hela細胞
CD42011-08-29 WBIF IHCIP
BECTONx50
mouse
Mono, IgG1

Hela細胞
CD4-biotin2011-08-29 SK3WBIF IHCIP
BECTONx50
mouse
mono, IgG1

Hela細胞
Clathrin2011-08-29 CHC5.9WBIF IHCIP
PROGEN
mouse
Mono, IgM
IgM
Hela細胞
Caveolin2011-08-29 clone 2297WBIF IHCIP
BD Transductionx100-
mouse
【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【saponin条件】0.05% saponin /PBS
Mono, IgG1
染まり方にムラがある。
Uneven staining
Hela細胞
Clathrin2011-08-29 X22WBIF IHCIP
ABRx500x1000x500
mouse
【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【saponin条件】0.05% saponin /PBS
Mono, IgG1
HeLaではだまが見える
Aggregates are observed in HeLa cells.
Hela細胞
CHC2011-08-29 clone 23WBIF IHCIP
BD Transductionx1000
mouse
Mono, IgG1

Hela細胞
CHC2011-08-29 TD.1WBIF IHCIP
Santa Cruzx1000x500
mouse
Mono, IgG1
WB感度よくない
Not sensitive for WB.
Hela細胞
CD632011-08-29 WBIF IHCIP
Ancellx1000
mouse
【PFA+Triton】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1

Hela細胞
mouse CD42011-08-10 clone: RM4-5WBIF IHCIP
BioLegendx100
100505
3%PFA (in PBS)
rat

マウス脾臓
mouse CD8a2011-08-10 clone: 53-6.7WBIF IHCIP
BioLegendx100
100701
3%PFA (in PBS)
rat

マウス脾臓
mouse CD11b2011-08-10 clone:M1/70WBIF IHCIP
BioLegendx100
101201
3%PFA (in PBS)
rat

マウス脾臓
mouse CD252011-08-10 clone: 3C7WBIF IHCIP
BioLegend
101901
rat

Biotin mouse CD282011-08-10 clone:37.51WBIF IHCIP
BioLegend
102103
hamster

mouse/human CD442011-08-10 clone:IM7WBIF IHCIP
BioLegend
103001
rat

human CD172011-08-10 clone: MEM-74WBIF IHCIP
Exbiox100
11215C100
3%PFA (in PBS), methanol
mouse
m

マウス脾臓
mouse CD11c2011-08-10 clone:N418WBIF IHCIP
BioLegend
117301
hamster

mouse CD45R/B2202011-08-10 WBIF IHCIP
BD Biosciencesx100
557390
3%PFA (in PBS)
rat
m

マウス脾臓
FITC mouse CD192011-08-10 WBIF IHCIP
BD Biosciences
557398
rat

murine CD362011-08-10 clone 63WBIF IHCIP
Cascade BioSx100
ABM5525
3%PFA (in PBS)
mouse
m
IgA抗体なので2次抗体に注意
This Ab is IgA, so use anti-IgA antibody for 2nd Ab.
マウス腹腔マクロファージ
murine CD44var2011-08-10 v6WBIF IHCIP
Bender MedSx100
BMS145
3%PFA (in PBS)
rat
m

マウス小腸
calnexin2012-06-21 WBIF IHCIP
BD Transduction Labx100
C45520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
calnexin2012-06-21 WBIF IHCIP
BD Transduction Labx100
C45520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
human clathrin heavy chain2011-08-12 WBIF IHCIP
Affinity BioR
MA1065
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human clathrin heavy chain2012-08-03 WBIF IHCIP
Affinity BioRx100
MA1065
3%PFA in PBS
mouse
0.05% saponin

PC12
L12012-08-03 WBIF IHCIP
Chemiconx100
MAB5272
3%PFA in PBS
rat
0.05% saponin

PC12
CDw17 AB-12011-08-10 clone:HuLy-m13WBIF IHCIP
Thermo FSx100
MS-522-P
3%PFA (in PBS), methanol
mouse
m

マウス脾臓
Rat IgMαCD772011-08-10 WBIF IHCIP
Santa Cruz
SC-59113
rat
m

caveolin-12011-08-10 N-20WBIF IHCIP
Santa Cruzx100
sc894
3%PFA (in PBS)
rabbit
p
平滑筋が染まる
Stains smooth muscles
マウス小腸

【D】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Dynamin II2011-08-29 27WBIF IHCIP
BD Transdutionx250
mouse
Mono, IgG2a

Hela細胞
Dynamin2011-08-29 41WBIF IHCIP
BD Transdutionx1000
mouse
Mono, IgG1

Hela細胞
DPPIV2012-04-04 HBB3/775/42 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG1
culture sup
Caco2
JCB 101, 838-851, 1985
mouse DPPIV2011-08-10 CD26 ectodomainWBIF IHCIP
R&D Systemsx100
AF954
3%PFA (in PBS)
goat

マウス小腸
human DPPIV2011-08-10 CD26 H-270WBIF IHCIP
Santa Cruzx100
sc9153
3%PFA (in PBS)
rabbit
p

マウス小腸

【E】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
E-Cadherin2011-08-29 36WBIF IHCIP
BD Transductionx2500x200-500
mouse
Mono, IgG2a

Hela細胞
EGFR2011-09-27 WBIF IHCIP
Fitzgerald1:2000
20-ES04
sheep
p
human専用
EGF receptor activated2011-09-27 WBIF IHCIP
BD500
610025
mouse
m
1%PFA, 4%PFA, MeOH, TCA
A431
L1 (8D9)2012-08-03 WBIF IHCIP
DSHBx100
8D9
3%PFA in PBS
mouse
0.05% saponin

PC12
mouse EphB32011-08-10 WBIF IHCIP
R&D Systemsx100
AF432
3%PFA (in PBS)
goat

マウス小腸
mouse Ephrin-B12011-08-10 WBIF IHCIP
R&D Systemsx100
AF473
3%PFA (in PBS)
goat

マウス小腸
Epidermal Growth Factor Receptor2011-09-27 clone 29.1WBIF IHCIP
Sigma500
E2760
mouse
m
1%PFA, 4%PFA, TCA
A431
ERp722012-06-21 WBIF IHCIP
BD Transduction Labx100
E72920
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
ERp722012-06-21 WBIF IHCIP
BD Transduction Labx100
E72920
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK

【G】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
human GLUT-12011-08-10 WBIF IHCIP
IBLx100
18901
3%PFA (in PBS), methanol
rabbit

マウス小腸
human GLUT-32011-08-10 WBIF IHCIP
IBLx100
18903
3%PFA (in PBS), methanol
rabbit

マウス小腸
human GLUT-52011-08-10 WBIF IHCIP
IBLx100
18905
3%PFA (in PBS), methanol
rabbit

マウス小腸
GM12011-08-10 clone:GMB16WBIF IHCIP
Seikagakux100
370685
3%PFA (in PBS), methanol
mouse

マウス小腸
GM32011-08-10 clone:GMR6WBIF IHCIP
Seikagakux100
370695
3%PFA (in PBS), methanol
mouse

マウス小腸
GM32011-08-10 M2590WBIF IHCIP
日本バイオテスト研究所 Nippon Bio-test Laboratoriesx100
?
3%PFA (in PBS), methanol
mouse
m

マウス小腸
mouse galectin-42011-08-10 WBIF IHCIP
R&D Systems
AF2128
3%PFA (in PBS)
goat
(diffuse stain)
IFOK?
マウス小腸
GM1302012-05-21 WBIF IHCIP
BD Transduction Labx100
G65120
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
GM1302012-05-21 WBIF IHCIP
BD Transduction Labx100
G65120
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
GS282012-05-21 WBIF IHCIP
BD Transduction Labx100
G83820
3%PFA in PBS
mouse
0.05% saponin
IgG2a
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
GS282012-05-21 WBIF IHCIP
BD Transduction Labx100
G83820
3%PFA in PBS
mouse
0.05% saponin
IgG2a
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
golgin842012-05-21 WBIF IHCIP
BD Transduction Labx100
G93720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
golgin842012-05-21 WBIF IHCIP
BD Transduction Labx100
G93720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
mouse GLUT-22011-08-10 WBIF IHCIP
R&D Systemsx100
MAB1440
3%PFA (in PBS), methanol
rat
m

マウス小腸
Galpha-i2014-06-04 WBIF IHCIP
Santa Cruz Biotechnologyx20
sc-56536
4%FA in PHEM
mouse
IgG
Use TBS instead PBS as buffer.
HeLa
Kiyomitsu and Cheeseman, NCB 2012

【H】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
bovine MPR (22d4)2012-08-03 WBIF IHCIP
DSHBx100
22d4
3%PFA in PBS
mouse
0.05% saponin

PC12
bovine MPR (86f7)2012-08-03 WBIF IHCIP
DSHBx100
86f7
3%PFA in PBS
mouse
0.05% saponin

PC12
human SNAP-252012-08-03 WBIF IHCIP
Chemiconx100
AB5871P
3%PFA in PBS
goat
0.05% saponin

PC12
mouse LAMP2 (ABL93)2012-08-03 WBIF IHCIP
DSHBx100
ABL93
3%PFA in PBS
rat
0.05% saponin

PC12
Hsp902012-06-21 WBIF IHCIP
BD Transduction Labx100
H38220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
Hsp902012-06-21 WBIF IHCIP
BD Transduction Labx100
H38220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
Hsp1102012-06-21 WBIF IHCIP
BD Transduction Labx100
H44620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
Hsp1102012-06-21 WBIF IHCIP
BD Transduction Labx100
H44620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
Hsp702012-06-21 WBIF IHCIP
BD Transduction Labx100
H53220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
Hsp702012-06-21 WBIF IHCIP
BD Transduction Labx100
H53220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
HSF42012-06-21 WBIF IHCIP
BD Transduction Labx100
H65520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
HSF42012-06-21 WBIF IHCIP
BD Transduction Labx100
H65520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
H/K ATPase2011-08-14 WBIF IHCIP
ABRx100
MA3-923
3%PFA (in PBS)
mouse
m

マウス胃
H+/K+ ATPase beta2011-08-10 WBIF IHCIP
Affinity BioRx100
MA3923
3%PFA (in PBS)
mouse
m

マウス胃

【I】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
IL-1 receptor2011-08-29 12A6WBIF IHCIP
Pharmingenx200-500
rat
Mono
mouseCD121a, TypeI/p80
Hela細胞

【K】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
KDEL2012-08-03 WBIF IHCIP
Stressgenx100
SPA827
3%PFA in PBS
mouse
0.05% saponin

PC12

【L】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
LDLR2011-08-29 C7WBIF IHCIP
ATCCダメ(?)x100
mouse
【PFA+Triton】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
mono
Hybridoma Supernatant
Hela細胞
FITC-Lotus2011-08-10 WBIF IHCIP
ホーネン  J-Oil Millsx100
300219
3%PFA (in PBS)
レクチン

マウス腎臓
L12011-08-10 8D9WBIF IHCIP
DSHBx100
8D9
3%PFA (in PBS)
mouse

マウス脳
LGN2014-06-04 WBIF IHCIP
Bethyl Laboratoriesx1000
A303-032A
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, Cell 2013
L12011-08-10 WBIF IHCIP
Chemiconx100
MAB5272
3%PFA (in PBS)
rat
m

マウス脳

【M】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
MHCI2011-08-29 W6/32WBIF IHCIP
ATCC原液orx10
mouse
【PFA+Triton】:△,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono
Hybridoma Sup't、Chase後、Fixする前に必ずAcid washすること。
After chase, wash with acid before fix.
Hela細胞
maltase2012-04-04 HBB2/143/17 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG1
culture sup
Caco2
JCB 101, 838-851, 1985
MDR1+32011-08-10 clone:C219WBIF IHCIP
Zymedx100
18-7244
methanol
mouse
メタノール固定でOK
Good staining by methanol fix.
マウス肝臓
MRP22011-08-10 M2?-5)=MDR2WBIF IHCIP
Alexis
ALX-801-037
mouse
m

mouse metabtropic GluR1a2011-08-10 WBIF IHCIP
Frontierx100
mGluR1aGoAf12201
3%PFA (in PBS)
goat
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
mouse metabtropic GluR1a2011-08-10 WBIF IHCIP
Frontierx100
mGluR1aGoAf12201
3%PFA (in PBS)
goat
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳

【N】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
NaK ATPase αsubunit2012-04-04 α5 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx50
3%PFA in PBS
mouse
0.05% saponin
IgG1
concentrated culture sup
Caco2
EMBO J 8, 193-202
NaK ATPase α1subunit2012-04-04 α6F monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx50
3%PFA in PBS
mouse
0.05% saponin
IgG2a
concentrated culture sup
Caco2
JBC 263, 4347-54, 1988

【n】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Na+K+ ATPase2015-01-13 WBIF IHCIP
Upstate biotechnologyx100
05-369
3%PFA (in PBS)
Mouse
IgG1κ

mouse kidney frozen section
Na+K+ ATPase2015-02-27 WBIF IHCIP
Upstate biotechnologyx100
05-369
3%PFA (in PBS)
Mouse
IgG1κ

mouse kidney frozen section

【N】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
rabbit Na+/K+ ATPase a-12011-08-10 WBIF IHCIP
Upstatex100
5369
3%PFA (in PBS)
mouse
m

マウス小腸
NMDAR2A2011-08-10 WBIF IHCIP
Millipore
AB1555P
3%PFA (in PBS)
rabbit
p

IFOK?
マウス脳
goatαNotch12011-08-10 M-20)WBIF IHCIP
Santa Cruz
sc-6015
goat
p

【NU】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
4.1R/EPB412014-06-04 WBIF IHCIP
Protein Tech Groupx1000
13014-1-AP
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, Cell 2013
4.1G/EPB41L2014-06-04 WBIF IHCIP
Bethyl Laboratoriesx500x500
A301-424A
3%PFA (in PBS), 4%FA in PHEM
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, Cell 2013

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
NGF-receptor (p75)2012-08-03 WBIF IHCIP
NeoMarkersx100
3%PFA in PBS
mouse
0.05% saponin

PC12
human podocalyxin2011-08-10 WBIF IHCIP
R&D Systemsx100
AF1658
methanol
goat
メタノール固定で弱く染まる?
Weak staining by methanol fix.
MDCK
human p75 pAb2012-08-03 WBIF IHCIP
Promegax100
G3231
3%PFA in PBS
rabbit
0.05% saponin

PC12
p230tranGolgi2012-05-21 WBIF IHCIP
BD Transduction Labx100
G88620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
p1152012-05-21 WBIF IHCIP
BD Transduction Labx100
P67420
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
PDI2012-06-21 WBIF IHCIP
BD Transduction Labx100
P71720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
PDI2012-06-21 WBIF IHCIP
BD Transduction Labx100
P71720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Rab82012-04-17 4/Rab4WBIF IHCIP
Transductionx5,000~
61-844
mouse
IgG2b

sheep Red Blood Cells2011-08-10 SRBCWBIF IHCIP
ICTx100
A6840
3%PFA (in PBS)
rabbit

羊赤血球
rat ROMK12011-08-10 Kir1.1, Kcnj1WBIF IHCIP
Alomonex100
APC001
3%PFA (in PBS)
rabbit

マウス腎臓
rat ROMK12011-08-10 Kir1.1, Kcnj1WBIF IHCIP
Alomonex100
APC001
3%PFA (in PBS)
rabbit

マウス腎臓
Rab82012-05-21 WBIF IHCIP
BD Transduction Labx100
R66320
3%PFA in PBS
mouse
0.05% saponin
IgG2b
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
Rab82012-05-21 WBIF IHCIP
BD Transduction Labx100
R66320
3%PFA in PBS
mouse
0.05% saponin
IgG2b
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
Rab82012-04-17 WBIF IHCIP
SantaCrusx500-1,000
sc-26578
goat

Rab6A2012-04-17 38-TBWBIF IHCIP
SantaCruzx1,000
sc-81913
mouse

human Rab6 (C-19)2012-08-03 WBIF IHCIP
Santa Cruzx100
sc310
3%PFA in PBS
rabbit
0.05% saponin

PC12

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
sucrase-isomaltase2012-04-04 HBB2/614/88 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG1
culture sup
Caco2
JCB 101, 838-851, 1985
sucrase-isomaltase2012-04-04 HBB2/219/20 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG2b
culture sup
Caco2
JCB 101, 838-851, 1985
SGLT-12011-08-14 WBIF IHCIP
CHEMICONx100
AB1352
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
Sodium Potassium ATPase2011-09-27
About Product
MonoclonalWBIF IHCIP
Abcam200-500
ab7671
mouse
IgG1
4%PFA, 1%PFA, TCA
DLD?1
syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma5000
S0664
mouse

pancreas lysate
syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma100
S0664
3%PFA (in PBS)
mouse

mouse primary neuron, neural progenitor cell, pancreas
syntaxin62012-05-21 WBIF IHCIP
BD Transduction Labx100
S55420
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
syntaxin62012-05-21 WBIF IHCIP
BD Transduction Labx100
S55420
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
STI-12012-06-21 WBIF IHCIP
BD Transduction Labx100
S65720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
STI-12012-06-21 WBIF IHCIP
BD Transduction Labx100
S65720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
mouse SGLT-12011-08-10 M-19WBIF IHCIP
Santa Cruzx100
sc20582
3%PFA (in PBS)
goat
p

マウス小腸
SV22012-08-03 WBIF IHCIP
DSHBx100
SV2
3%PFA in PBS
mouse
0.05% saponin

PC12

【T】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
TGN462011-08-29 WBIF IHCIP
Cosmo biox200
sheep
【PFA+Triton】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Poly
2ndはDonkey anti-sheep Cy3を使用、MDCKIIダメ
Use Cy3 labeled donkey anti-sheep as 2nd Ab. Not suitable for MDCKII.
Hela細胞
TfnR2011-08-29 H68.4WBIF IHCIP
Zymedx1000-x5000-
mouse
【PFA+Triton】:○,【PFA+Saponin】:○,【TCA】:○【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1
Human OK, MDCKII OK, OverexpressionしているArfraptin2を認識する。
Recognize overexpressed Arfaptin2.
Hela細胞
TLR42011-08-29 HTA125WBIF IHCIP
IMGENEXx500x200
mouse
Mono, IgG2a

Hela細胞
FITC mouse TCR-b chain2011-08-10 WBIF IHCIP
BioLegend
109205
hamster

human Transferrin Receptor2012-08-03 WBIF IHCIP
Zymedx100
136800
3%PFA in PBS
mouse
0.05% saponin

PC12
Biotin mouse CD712011-08-10 TFRWBIF IHCIP
BD Pharmingenx100
557416
3%PFA (in PBS), methanol
rat
m

マウス小腸
Tfn (HRP)2011-08-29
About Product
MonoclonalWBIF IHCIP
Abcam
ab74415
mouse
IgG1
x24000 For ELISA
Hela細胞
rat TGN382012-08-03 WBIF IHCIP
Serotecx100
AHP499G
3%PFA in PBS
sheep
0.05% saponin

PC12
human tamm-horsfall glycoprotein2011-08-10 WBIF IHCIP
Biocedical Tx100
BT590
3%PFA (in PBS)
rabbit

マウス腎臓
TfnR(CD71)2011-08-29 CD71WBIF IHCIP
SIGMAx200-
C2063
mouse
【PFA+Triton】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1
Lumen側を認識する抗体
Ab recognize luminal part.
Hela細胞

【V】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
VAMP3 (cellubrevin)2015-06-02 WBIF IHCIP
synaptic systemsx1000x100
104102
3%PFA (in PBS)
rabbit
For WB, 20ug of neural progenitor cell lysate was used
mouse primary neuron, neural progenitor cell
synaptobrevin/VAMP 22012-08-03 WBIF IHCIP
Synaptic Syx100
104211
3%PFA in PBS
mouse
0.05% saponin

PC12
Vti1a2012-05-21 WBIF IHCIP
BD Transduction Labx100
V85620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
Vti1a2012-05-21 WBIF IHCIP
BD Transduction Labx100
V85620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK